全部商品分类
用小程序，查商品更便捷
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser169 of human PANK2 protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
Specificity/Sensitivity
Species Reactivity:
Human
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
参考图片
Simple WesternTM analysis of lysates (0.1 mg/mL) from Raji cells using Phospho-PANK2 (Ser169) (F7O5Q) Rabbit mAb #88224. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Western blot analysis of extracts from MCF7 cells, serum-starved and untreated (-) or treated with human IGF-I (hIGF-I) (100 ng/mL, 20 min; +) that were subsequently untreated (-) or treated with λ phosphatase and calf intestinal phosphatase (CIP) (+), using Phospho-PANK2 (Ser169) (F7O5Q) Rabbit mAb (upper), a PANK2 antibody (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from SK-OV-3 and Raji cells, untreated (-) or treated with λ phosphatase and calf intestinal phosphatase (CIP) (+), using Phospho-PANK2 (Ser169) (F7O5Q) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).