OX40L (D6K7R) & CO-0087-750 SignalStar ™ Oligo-Antibody Pair

1/8

OX40L (D6K7R) & CO-0087-750 SignalStar ™ Oligo-Antibody Pair

品牌： CST

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

产品介绍

产品信息

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala15 of human OX40L protein.

简单描述

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

商品描述

Product Usage Information

Application	Dilution
SignalStar™ Leica Bond	1:50 - 1:200
SignalStar™ Manual	1:50 - 1:200

应用

目标/特异性

Specificity/Sensitivity

OX40L (D6K7R) Rabbit mAb (SignalStar™ Conjugate 0087) recognizes endogenous levels of total OX40L protein. Staining of unknown specificity has been observed in skeletal muscle.

Species Reactivity:

Human

背景

OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12). 1.Croft, M. (2010) Annu Rev Immunol 28, 57-78. 2.So, T. and Croft, M. (2012) Front Immunol 3, 133. 3.Paterson, D.J. et al. (1987) Mol Immunol 24, 1281-90. 4.Mallett, S. et al. (1990) EMBO J 9, 1063-8. 5.Dürkop, H. et al. (1995) Br J Haematol 91, 927-31. 6.Godfrey, W.R. et al. (1994) J Exp Med 180, 757-62. 7.Al-Shamkhani, A. et al. (1997) J Biol Chem 272, 5275-82. 8.So, T. et al. (2011) Proc Natl Acad Sci U S A 108, 2903-8. 9.So, T. and Croft, M. (2013) Front Immunol 4, 139. 10.Gough, M.J. and Weinberg, A.D. (2009) Adv Exp Med Biol 647, 94-107. 11.Weinberg, A.D. et al. (2011) Immunol Rev 244, 218-31. 12.Linch, S.N. et al. (2015) Front Oncol 5, 34.

制备和贮存

保存方式

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

数据库链接

Entrez-Gene ID

7292

UniProt ID

P23510

参考图片

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human tonsil using PCNA (D3H8P) & CO-0090-488 SignalStar™ Oligo-Antibody Pair #36181 (magenta), Na,K-ATPase α1 (D4Y7E) & CO-0079-594 SignalStar™ Oligo-Antibody Pair #85191 (cyan), and OX40L (D6K7R) & CO-0087-647 SignalStar™ Oligo-Antibody Pair #73292 (red). All fluorophores have been assigned a pseudocolor, as indicated.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor using PCNA (D3H8P) & CO-0090-594 SignalStar™ Oligo-Antibody Pair #49873 (green), Na,K-ATPase α1 (D4Y7E) & CO-0079-647 SignalStar™ Oligo-Antibody Pair #16129 (magenta), and OX40L (D6K7R) & CO-0087-750 SignalStar™ Oligo-Antibody Pair #95054 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using OX40L (D6K7R) & CO-0087-488 SignalStar™ Oligo-Antibody Pair #49516 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using OX40L (D6K7R) & CO-0087-594 SignalStar™ Oligo-Antibody Pair #58323 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using OX40L (D6K7R) & CO-0087-647 SignalStar™ Oligo-Antibody Pair #73292 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor using OX40L (D6K7R) & CO-0087-750 SignalStar™ Oligo-Antibody Pair #95054 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human melanoma using OX40L (D6K7R) & CO-0087-647 SignalStar™ Oligo-Antibody Pair #73292 (left, red) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human melanoma using OX40L (D6K7R) Rabbit mAb #59036 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using OX40L (D6K7R) & CO-0087-488 SignalStar™ Oligo-Antibody Pair #49516 (green), CD14 (D7A2T) & CO-0085-594 SignalStar™ Oligo-Antibody Pair #74041 (yellow), PCNA (D3H8P) & CO-0090-647 SignalStar™ Oligo-Antibody Pair #60626 (cyan), MHC Class I (EMR8-5) & CO-0082-750 SignalStar™ Oligo-Antibody Pair #55370 (magenta), CD141/Thrombomodulin (E7Y9P) & CO-0088-488 SignalStar™ Oligo-Antibody Pair #82890 (red), and CD8α (D4W2Z) & CO-0040-647 SignalStar™ Oligo-Antibody Pair #57623 (white). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

95054S

一键复制

询价

1Kit

选择数量

当前规格1件起购

配送至

预计2-3周送达，快递: 免运费，若需干冰额外收费

询价大包装询价