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IL-2Ralpha/CD25(E9W2J)CO-0074-647 SignalStar Oligo-Antibody

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IL-2Ralpha/CD25(E9W2J)CO-0074-647 SignalStar Oligo-Antibody

品牌： CST

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产品介绍

产品信息

来源纯化

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse IL-2Rα/CD25 protein.

简单描述

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

商品描述

Product Usage Information

Application	Dilution
SignalStar™ Leica Bond	1:50 - 1:200
SignalStar™ Manual	1:50 - 1:200

应用

目标/特异性

Specificity/Sensitivity

IL-2Rα/CD25 (E9W2J) XP Rabbit mAb (SignalStar™ Conjugate 0074) recognizes endogenous levels of total IL-2Rα/CD25 protein. This antibody non-specifically labels myenteric nerves in the gut.

Species Reactivity:

Mouse

背景

Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2). 1.Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79. 2.Gaffen, S.L. and Liu, K.D. (2004) Cytokine 28, 109-23. 3.Fehérvari, Z. et al. (2006) Trends Immunol 27, 109-11. 4.Antony, P.A. et al. (2006) J Immunol 176, 5255-66. 5.Jaleco, S. et al. (2003) J Immunol 171, 61-8.

制备和贮存

保存方式

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

数据库链接

Entrez-Gene ID

16184

UniProt ID

P01590

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货号：

58696S

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询价

1 Kit

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