Granzyme F (E2U7L) Rabbit mAb (Alexa Fluor ® 488 Conjugate)

Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). In mice, ten family members have been identified: A, B, C, D, E, F, G, K, M, and N (2). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (3). Granzyme F induces a novel cell death pathway consisting of externalization of phosphatidylserine (PS), nuclear condensation, mitochondrial damage, cytochrome c release, single-stranded DNA nicking, and caspase inactivation (4). CTLs from mice with reduced expression of Granzyme F and C display diminished cytotoxicity (5). 1.Trapani, J.A. (2001) Genome Biol 2, REVIEWS3014. 2.Russell, J.H. and Ley, T.J. (2002) Annu Rev Immunol 20, 323-70. 3.Lord, S.J. et al. (2003) Immunol Rev 193, 31-8. 4.Shi, L. et al. (2009) Cell Death Differ 16, 1694-706. 5.Getachew, Y. et al. (2008) J Immunol 181, 7810-7.