E-Cadherin (24E10) & CO-0103-488 SignalStar ™ Oligo-Antibody Pair

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E-Cadherin (24E10) & CO-0103-488 SignalStar ™ Oligo-Antibody Pair

品牌： CST

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产品介绍

产品信息

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein.

简单描述

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

商品描述

Product Usage Information

Application	Dilution
SignalStar™ Leica Bond	1:50 - 1:200
SignalStar™ Manual	1:50 - 1:200

应用

目标/特异性

Specificity/Sensitivity

E-Cadherin (24E10) Rabbit mAb (SignalStar™ Conjugate 0103) detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin.

Species Reactivity:

Human, Mouse

背景

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). 1.Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35. 2.Christofori, G. (2003) EMBO J 22, 2318-23. 3.Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63. 4.Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34. 5.Rabascio, C. et al. (2004) Cancer Res 64, 4373-7. 6.Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7. 7.Patel, I.S. et al. (2003) Int J Cancer 106, 172-7. 8.Sanders, D.S. et al. (2000) J Pathol 190, 526-30.

制备和贮存

保存方式

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

数据库链接

Entrez-Gene ID

999

UniProt ID

P12830

参考图片

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using MHC Class II (LGII-612.14) & CO-0105-488 SignalStar™ Oligo-Antibody Pair #55436 (green), E-Cadherin (24E10) & CO-0103-594 SignalStar™ Oligo-Antibody Pair #65400 (magenta), CD28 (D2Z4E) & CO-0081-647 SignalStar™ Oligo-Antibody Pair #96977 (red), α-Smooth Muscle Actin (D4K9N) & CO-0024-488 SignalStar™ Oligo-Antibody Pair #76133 (green), TCF1/TCF7 (C63D9) & CO-0006-594 SignalStar™ Oligo-Antibody Pair #34706 (yellow), and Arginase-1 (D4E3M) & CO-0075-647 SignalStar™ Oligo-Antibody Pair #44900 (white). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using E-Cadherin (24E10) & CO-0103-488 SignalStar™ Oligo-Antibody Pair #48503 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using E-Cadherin (24E10) & CO-0103-594 SignalStar™ Oligo-Antibody Pair #65400 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using E-Cadherin (24E10) & CO-0103-647 SignalStar™ Oligo-Antibody Pair #31774 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using E-Cadherin (24E10) & CO-0103-750 SignalStar™ Oligo-Antibody Pair #65506 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using E-Cadherin (24E10) & CO-0103-750 SignalStar™ Oligo-Antibody Pair #65506 (left, cyan) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human squamous cell lung carcinoma using E-Cadherin (24E10) Rabbit mAb #3195 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse thymus using CD28 (D2Z4E) & CO-0081-488 SignalStar™ Oligo-Antibody Pair #52935 (green), and E-Cadherin (24E10) & CO-0103-647 SignalStar™ Oligo-Antibody Pair #31774 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.

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货号：

48503S

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