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咨询Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.
SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Product Usage Information
| Application | Dilution |
|---|---|
| SignalStar™ Leica Bond | 1:50 - 1:200 |
| SignalStar™ Manual | 1:50 - 1:200 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse
SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.
参考图片
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using c-Fos (E2I7R) & CO-0131-488 SignalStar™ Oligo-Antibody Pair #28962 (green), Phospho-CREB (Ser133) (87G3) & CO-0125-594 SignalStar™ Oligo-Antibody Pair #34820 (yellow), and TMEM119 (E3E4T) & CO-00130-647 SignalStar™ Oligo-Antibody Pair #89700 (red). All fluorophores have been assigned a pseudocolor, as indicated.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using c-Fos (E2I7R) & CO-0131-488 SignalStar™ Oligo-Antibody Pair #28962 (green), α-Smooth Muscle Actin (D4K9N) & CO-0024-594 SignalStar™ Oligo-Antibody Pair #25040 (magenta), and Phospho-CREB (Ser133) (87G3) & CO-0125-647 SignalStar™ Oligo-Antibody Pair #84641 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse brain using Phospho-CREB (Ser133) (87G3) & CO-0125-488 SignalStar™ Oligo-Antibody Pair #71574 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Phospho-CREB (Ser133) (87G3) & CO-0125-594 SignalStar™ Oligo-Antibody Pair #34820 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using Phospho-CREB (Ser133) (87G3) & CO-0125-647 SignalStar™ Oligo-Antibody Pair #84641 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human cerebellum using Phospho-CREB (Ser133) (87G3) & CO-0125-750 SignalStar™ Oligo-Antibody Pair #97252 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Phospho-CREB (Ser133) (87G3) & CO-0125-594 SignalStar™ Oligo-Antibody Pair #34820 (left, yellow) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human colon adenocarcinoma using Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human soft tissue squamous cell carcinoma using Phospho-CREB (Ser133) (87G3) & CO-0125-488 SignalStar™ Oligo-Antibody Pair #71574 (green), TMEM119 (E3E4T) & CO-00130-647 SignalStar™ Oligo-Antibody Pair #89700 (red), c-Fos (E2I7R) & CO-0131-750 SignalStar™ Oligo-Antibody Pair #85602 (cyan), Pan-Keratin (C11) & CO-0003-488 SignalStar™ Oligo-Antibody Pair #63566 (magenta), CD68 (D4B9C) & CO-0007-594 SignalStar™ Oligo-Antibody Pair 77318 (yellow), CD8α (D8A8Y) & CO-0004-750 SignalStar™Oligo-Antibody Pair #62750 (orange), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.