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产品详细信息
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
靶标信息
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ATP receptor; ATP-gated cation channel protein; ionotropic purinergic receptor; P2X purinoceptor 4; P2X receptor, subunit 4; P2X4; Purinergic receptor; purinergic receptor P2X, ligand gated ion channel, 4; purinergic receptor P2X, ligand-gated ion channel, 4; purinergic receptor P2X4; purinergic receptor P2x4 subunit variant e; purinoceptor P2X4
基因别名: AI504491; AW555605; D5Ertd444e; P2RX4; P2X4; P2X4R
UniProt ID:(Human) Q99571, (Rat) P51577
Entrez Gene ID:(Human) 5025, (Mouse) 18438, (Rat) 29659
参考图片
Expression of P2RX4 in rat pheochromocytoma cell line (PC12) - Cell surface detection of P2RX4 in intact living PC12 cells. A. Extracellular staining of cells with Anti-P2X4 (extracellular) Antibody (#APR-024), (red, 1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . B. Nuclear staining of cells using DAPI (blue). C. Merged images of A and B.
Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), C6 rat brain glioma cell line lysate (lanes 3 and 7) and SH-SY5Y human brain neuroblastoma cell line lysate (lanes 4 and 8): - 1-4. Anti-P2X4 Receptor (extracellular) Antibody (#APR-024), (1:200).5-8. Anti-P2X4 Receptor (extracellular) Antibody , preincubated with P2X4 Receptor (extracellular) Blocking Peptide (#BLP-PR024).
Cell surface detection ofP2X4by indirect flow cytometry in live intacthuman THP-1monocyticleukemiacells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-P2X4 Receptor (extracellular) Antibody &(8)239; (#APR-024), (2.5μg) + goat- Anti-rabbit-FITC.