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TRPM8 (EXTRACELLULAR) ANTIBODY
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产品详细信息
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
靶标信息
TRPM8 is a receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. TRPM8 is activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH and involved in menthol sensation. TRPM8 is permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. TRPM8 has an expression pattern within normal human tissues that is mostly restricted to prostate epithelial cells. TRPM8 mRNA expression is at moderate levels in normal prostate tissue and appears to be elevated in prostate cancer. Notably, TRPM8 mRNA was also expressed in a number of nonprostatic primary tumors of breast, colon, lung, and skin origin, whereas transcripts encoding TRPM8 were hardly detected or not detected in the corresponding normal human tissues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cold menthol receptor 1; cold/menthol receptor 1; Long transient receptor potential channel 6; LTrpC-6; MGC2849; short form of the TRPM8 cationic channel; transient receptor melastatin 8 variant 1; transient receptor melastatin 8 variant 2; Transient receptor potential cation channel subfamily M member 8; transient receptor potential cation channel, subfamily M, member 8; Transient receptor potential p8; transient receptor potential subfamily M member 8; transient receptor potential-p8; TRP ion channel TRPM8; Trp-p8
基因别名: CMR1; LTrpC-6; LTRPC6; Trp-p8; TRPM8; TRPP8
UniProt ID:(Human) Q7Z2W7, (Rat) Q8R455, (Mouse) Q8R4D5
Entrez Gene ID:(Human) 79054, (Rat) 171384, (Mouse) 171382
参考图片
Expression of TRPM8 in rat DRG cells - Immunocytochemistry of rat dorsal root ganglion (DRG) cells. A. Intracellular staining of cells with Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:500) followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody . B. Extracellular staining of live cells with Anti-TRPM8 (extracellular) Antibody (1:50) followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody . The cell-permeable dye Hoechst 33342 (blue) was used for nuclear staining.
Expression of TRPM8 in LNCaP prostate carcinoma cell line - Cell surface detection of TRPM8 in LNCaP cells with Anti-TRPM8 (extracellular) Antibody (#ACC-049). A. Extracellular staining of intact LNCaP cells with Anti-TRPM8 (extracellular) Antibody (1:100), followed by goat Anti-rabbit-AlexaFluor-550 (red), (x100). B. Intracellular staining of LNCaP cells with Anti-TRPM8 (extracellular) Antibody (1:1000), followed by goat Anti-rabbit-AlexaFluor-488 (green), (x100).
Expression of TRPM8 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:100). TRPM8 is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.
Multiplex staining of TRPM8 and TrkA in rat DRG - Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody (#ANT-018-FR), (1:60) and Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:300). A. TRPM8 labeling followed by goat- Anti-rabbit-Alexa-488 (green). B. The same section was then labeled for TrkA (purple). C. Merge of A and B demonstrates co-localization of TRPM8 and TrkA in rat DRG (arrows). Cell nuclei were stained with DAPI (blue).
Western blot analysis of prostate carcinoma cell lines DU145 (lanes 1 and 3),Human LNCaP prostate carcinoma (lanes 2 and 4) and mouse-TRPM8 transfected HEK-293 (lanes 5 and 6) cell lysates: - 1,2,5. Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:200).3,4,6. Anti-TRPM8 (extracellular) Antibody , preincubated with TRPM8 (extracellular) Blocking Peptide (#BLP-CC049).