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产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Glutamate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. Cross reactivity against Glutamate Dehydrogenase from other sources may occur but have not been specifically determined.
靶标信息
Glutamate dehydrogenase, GluD1, is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: GDH 1; Glutamate dehydrogenase 1, mitochondrial
基因别名: GDH; GDH 1; GDH1; GLUD; GLUD1
UniProt ID:(Bovine) P00366
Entrez Gene ID:(Bovine) 281785
参考图片
Dot Blot results of Rabbit Anti-Glutamate Dehydrogenase Biotin Conjugated. Antigen: Glutamate Dehydrogenase. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Glutamate Dehydrogenase Biotin 1µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP (S000-03) at 1:40,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.