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产品详细信息
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
靶标信息
This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: a4 nicotinic receptor; acetylcholine receptor; acetylcholine receptor alpha 4 neural; acetylcholine receptor, neuronal, nicotinic, alpha-4 subunit; alpha4 nAChR; alpha4-nAChR; cholinergic receptor; cholinergic receptor, nicotinic alpha 4; cholinergic receptor, nicotinic, alpha 4 (neuronal); cholinergic receptor, nicotinic, alpha 4 subunit; cholinergic receptor, nicotinic, alpha polypeptide 4; CHRNA 4; nAChR alpha 4; Neuronal acetylcholine receptor subunit alpha-4; Neuronal nicotinic acetylcholine receptor alpha 4 subunit; neuronal nicotinic acetylcholine receptor alpha-4 subunit
基因别名: Acra-4; Acra4; BFNC; CHRNA4; EBN; EBN1; ENFL1; NACHR; NACHRA4; NACRA4; NARAC
UniProt ID:(Human) P43681, (Mouse) O70174, (Rat) P09483
Entrez Gene ID:(Human) 1137, (Mouse) 11438, (Rat) 25590
参考图片
Multiplex staining of nAChR alpha 4 and nAChR beta 2 in mouse brain - Immunohistochemical staining of perfusion-fixed frozen mouse parietal cortex sections using Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:300) and Anti-Nicotinic Acetylcholine Receptor beta 2 (CHRNB2) (extracellular)-ATTO Fluor-594 Antibody (#ANC-012-AR), (1:60).A. nAChR alpha 4 staining (green). B. Same section stained for nAChR beta 2 (red). C. Merge of the two images reveals several cells expressing both receptors (arrows). Nuclei are stained with DAPI (blue).
Expression of nAChR alpha 4 in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:200). A. Expression of nAChR alpha 4 (green) was visualized in rat cerebellum. Purkinje cells were positive for nAChR alpha 4 (arrows). B. The same section was stained for Parvalbumin (red) and counterstained (blue).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates: - 1,2. Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:200).3,4. Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 (extracellular) Blocking Peptide (#BLP-NC004).
Cell surface detection ofnAChR alpha 4by indirect flow cytometry in live intacthumanJurkatT-cell leukemia cells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody &(8)239; (#ANC-004), (2.5μg) + goat- Anti-rabbit-FITC.