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NICOTINIC ACETYLCHOLINE RECEPT
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NICOTINIC ACETYLCHOLINE RECEPT

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品牌: Thermo
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应用
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已发表文章

免疫印迹 (WB)

1:400
-

免疫细胞化学 (ICC/IF)

1:50
-
产品规格

种属反应

Mouse,Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

(C)NAKGMKGNRREG, corresponding to amino acid residues 211-222 of rat nAChR beta 3, Extracellular, N-terminus

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).

靶标信息

The nicotinic acetylcholine receptors are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

参考图片

Expression of nAChR beta 3 in live intact rat PC12 cells - Cell surface detection of nAChR beta 3 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Nicotinic Acetylcholine Receptor beta 3 (CHRNB3) (extracellular) Antibody (#ANC-013), (1:50), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live view of the cells. C. Merge image of A and B.

Western blot analysis of rat brain membranes: - 1. Anti-Nicotinic Acetylcholine Receptor beta 3 (CHRNB3) (extracellular) Antibody (#ANC-013), (1:400). 2. Anti-Nicotinic Acetylcholine Receptor beta 3 (CHRNB3) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor beta 3/CHRNB3 (extracellular) Blocking Peptide (#BLP-NC013).

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货号:
ANC-013-200UL
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200UL
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