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产品详细信息
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
靶标信息
After binding acetylcholine, the ACHR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: acetylcholine receptor; acetylcholine receptor, nicotinic, alpha 2 (neuronal); cholinergic receptor; cholinergic receptor, nicotinic alpha 2; cholinergic receptor, nicotinic, alpha 2 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal); Neuronal acetylcholine receptor subunit alpha-2; nicotinic acetylcholine receptor
基因别名: ACHR; Acra-2; Acra2; BC011490; CHRNA2
UniProt ID:(Human) Q15822, (Rat) O08952, (Mouse) Q91X60
Entrez Gene ID:(Human) 1135, (Rat) 170945, (Mouse) 110902
参考图片
Expression of nAChR alpha 2 in rat PC12 pheochromocytoma cells - Cell surface detection of nAChR alpha 2 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of live cells with Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:50), followed by goat Anti-rabbit-AlexaFluor-594 (red). B. Live image of the cells. C. Merge of the two images.
Expression of nAChR alpha 2 inrat deep cerebellar nucleus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:100). Staining reveals expression of nAChR alpha 2 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus.DAPI is used as thecounterstain (blue).
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:400).3,4. Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate: - 1. Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:200). 2. Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002).