FastScan AsCpf1/Cas12a (Strain BV3L6 ) ELISA Kit

CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from Prevotella and Francisella) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1/Cas12a utilizes T-Rich protospacer-adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1/Cas12a bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2,4).AsCpf1/Cas12a (Strain BV3L6) is a Cpf1/Cas12a enzyme derived from Acidaminococcus sp. BV3L6 (5,6). 1.Cong, L. et al. (2013) Science 339, 819-23. 2.Zetsche, B. et al. (2015) Cell 163, 759-71. 3.Horvath, P. and Barrangou, R. (2010) Science 327, 167-70. 4.Zhang, Y. et al. (2017) Sci Adv 3, e1602814. 5.Zetsche, B. et al. (2015) Cell 163, 759-71. 6.Zhang, Y. et al. (2017) Sci Adv 3, e1602814.