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Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

IgG light chain

来源纯化

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

宿主

Mouse

商品描述

Product Usage Information

Recommended Antibody Dilution:1:2000

同种型

Mouse

应用

反应种属

Rabbit

目标/特异性

Specificity/Sensitivity

Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb recognizes the light chain of rabbit IgG, either in its native conformation or in its denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.

Species Reactivity:

Rabbit

背景

Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunoprecipitation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb as a secondary antibody will eliminate this problem.

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

参考图片

Immunoprecipitation of PRAS40 from untreated HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PRAS40 (D23C7) Rabbit mAb #2691. All lanes are probed with PRAS40 (D23C7) Rabbit mAb as the primary antibody. Secondary antibodies include Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb (left panel) and Anti-rabbit IgG, HRP-linked Antibody #7074 (right panel). For the left panel, the bound Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb was detected by Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.

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货号：

45262S

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100ul

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