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beta-Actin (13E5) Rabbit mAb

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beta-Actin (13E5) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

beta-Actin

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human β-actin protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunohistochemistry (Paraffin)	1:50 - 1:200
Immunofluorescence (Frozen)	1:100 - 1:400
Immunofluorescence (Immunocytochemistry)	1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)	1:100 - 1:400

同种型

Rabbit IgG

分子量

内毒素水平

仓鼠 , 鸡 , 犬 , 马

研究领域

癌症,细胞生物学,纤维化,神经科学,

应用

反应种属

Human,Mouse,Rat,Monkey,Bovine,Pig,

预测反应种属

Hamster,Chicken,Dog,Horse,

目标/特异性

Specificity/Sensitivity

β-Actin (13E5) Rabbit mAb detects endogenous levels of total β-actin protein. Depsite the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, β-Actin (13E5) Rabbit mAb #4970 does not cross-react with cytoplasmic γ-actin, or any other actin isoforms.

Species Reactivity:

Human, Mouse, Rat, Monkey, Bovine, Pig

敏感性

Endogenous

背景

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids (2). For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (3). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (3). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (4). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (5-7). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (7). 1.Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55. 2.Perrin, B.J. and Ervasti, J.M. (2010) Cytoskeleton (Hoboken) 67, 630-4. 3.Condeelis, J. (2001) Trends Cell Biol 11, 288-93. 4.Lim, Y.P. et al. (2004) Clin Cancer Res 10, 3980-7. 5.Kayalar, C. et al. (1996) Proc Natl Acad Sci U S A 93, 2234-8. 6.Communal, C. et al. (2002) Proc Natl Acad Sci U S A 99, 6252-6. 7.Du, J. et al. (2004) J Clin Invest 113, 115-23.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #93473.

数据库链接

Entrez-Gene ID

UniProt ID

P60709

参考图片

Flow cytometric analysis of HeLa cells using β-Actin (13E5) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of cell extracts from various cell lines using beta-Actin (13E5) Rabbit mAb.

Western blot analysis of recombinant Actin isoforms using β-Actin (13E5) Rabbit mAb (upper) and Pan-Actin Antibody #4968 (lower).

Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using ß-Actin (13E5) Rabbit mAb #4970. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Immunohistochemical analysis of paraffin-embedded human heart using beta-Actin (13E5) Rabbit mAb. Note the lack of staining of cardiac actin.

Immunohistochemical analysis of paraffin-embedded human leiomyoma using beta-Actin (13E5) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using β-actin (13E5) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using beta-Actin (13E5) Rabbit mAb in the presence of control peptide (left) or beta-Actin Blocking Peptide #1025 (right).

Immunohistochemical analysis of paraffin-embedded human skeletal muscle using beta-Actin (13E5) Rabbit mAb. Note the lack of staining of skeletal muscle actin.

Confocal immunofluorescent analysis of fixed, frozen mouse pancreas at low magnification (left) and high magnification (right) using β-Actin (13E5) Rabbit mAb #4970 (red) and DAPI #4083 (blue).

Confocal immunofluorescent analysis of COS-7 cells using β-Actin (13E5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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货号：

4970T

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