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Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)

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Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)

品牌： CST

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产品介绍

产品信息

荧光素标记

HRP

抗原名称

IgG

来源纯化

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

宿主

Mouse

简单描述

Affinity purified mouse anti-rabbit IgG (Conformation Specific) HRP Conjugate antibody.

商品描述

Product Usage Information

Recommended Antibody Dilution:1:2000

同种型

Mouse

应用

反应种属

Mouse

目标/特异性

Specificity/Sensitivity

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) recognizes the native rabbit IgG. This antibody may cross react with denatured IgG at high concentrations.

Species Reactivity:

Rabbit

背景

Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

参考图片

Immunoprecipitation of Beclin-1 from HeLa cells using Beclin-1 (D40C5) Rabbit mAb #3495 (lane 2). Lane 1 contains input control lysate. All lanes are probed with Beclin-1 (D40C5) Rabbit mAb #3495 as the primary antibody. Secondary antibodies include Anti-rabbit IgG, HRP-linked Antibody #7074 (panel A) and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 (panel B).

Immunoprecipitation of PRAS40 from HeLa cells using PRAS40 (D23C7) XP® Rabbit mAb #2691 (lane 2). Lane 1 contains input control lysate. All lanes are probed with PRAS40 (D23C7) Rabbit mAb #2691 as the primary antibody. Secondary antibodies include Anti-rabbit IgG, HRP-linked Antibody #7074 (panel A) and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 (panel B).

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货号：

5127S

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供应商现货

100ul

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