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When diluted in lysis buffer to a final concentration of 1X the Protease Inhibitor Cocktail prevents protein degradation by endogenous proteases present in whole cell extract. The 100X Protease Inhibitor Cocktail is a clear, colorless liquid.
1X Protease Inhibitor Cocktail: 1mM AEBSF800nM Aprotinin50μM Bestatin15μM E-6420μM Leupeptin10μM Pepstatin A
Product Usage Information
1. Briefly vortex the Protease Inhibitor Cocktail (100X) before use.2. Just prior to lysing cells, dilute the cocktail 1:100 in desired lysis buffer to obtain a 1X working concentration.
Store the undiluted 100X cocktail at 4ºC. Do not freeze.
参考图片
Western blot analysis of extracts from NIH/3T3 cells, prepared in lysis buffer in the absence of protease inhibitors (left) or with Protease Inhibitor Cocktail (100X) #5871 added (right), and incubated at 37ºC for the indicated time points, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695. In the absence of protease inhibitors, p44/42 MAPK signal fades within 6 hrs. after harvest, indicating protein degradation. In the presence of the protease inhibitor cocktail, the p44/42 MAPK degradation is slowed significantly and signal is still present at 48 hrs. following harvest.