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Alix (3A9) Mouse mAb
品牌： CST
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对比
咨询分子量：
95
反应种属：
Human,Mouse,Rat,Monkey,
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Alix
来源纯化
Monoclonal antibody is produced by immunizing animals with full-length recombinant human Alix protein.
宿主
Mouse
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
同种型
Mouse IgG1
分子量
95
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,
目标/特异性
Specificity/Sensitivity
Alix (3A9) Mouse mAb detects endogenous levels of total Alix protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
Alix, a phylogenetically conserved cytosolic scaffold protein, contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain (1,2). Originally identified as an ALG-2 (apoptosis-linked gene 2)-interacting protein involved in programmed cell death (3,4), Alix also regulates many other cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT (endosomal sorting complex required for transport) proteins, endophilins, and CIN85 (Cbl-interacting protein of 85 kDa) (5,6).
1.Mattei, S. et al. (2006) Eur. J. Cell Biol. 85, 925-936.
2.Odorizzi, G. (2006) J. Cell Sci. 119, 3025-3032.
3.Vito, P. et al. (1999) J. Biol. Chem. 274, 1533-1540.
4.Missotten, M. et al. (1999) Cell Death Differ. 6, 124-129.
5.Katoh, K. et al. (2003) J. Biol. Chem. 278, 39104-39113.
6.Sadoul, R. (2006) Biol. Cell 98, 69-77.
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
10015
UniProt ID
Q8WUM4
参考图片
Immunoprecipitation of Alix from HeLa cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is Alix (3A9) Mouse mAb, #2171. Western blot was performed using Alix (3A9) Mouse mAb.
Western blot analysis of cell extracts from various cell types using Alix (3A9) Mouse mAb.
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