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对比
咨询Protein A Magnetic Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein A is covalently coupled to a magnetic particle.
Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies. Beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017 or 12-Tube Magnetic Separation Rack #14654, which concentrates the beads to the side of the tube instead of the bottom. This eliminates centrifugation steps, minimizes sample loss, increases washing efficiency, and saves time.
The 1mL and 5mL size is enough material for 25 and 125 immunoprecipitations, respectively, when following our recommended protocol.
Product Specifications:
Bead Diameter: ~1.5 μm
Binding Capacity: > 0.2 μg Rabbit IgG/μl bead slurry
Specificity/Sensitivity
Species Reactivity:
All Species Expected
Supplied in PBS, 0.1% BSA, 0.004% EDTA and 0.075% sodium azide. Store at 4°C. This product is stable for 12 months.
Do not freeze, dry or centrifuge beads. This may cause irreversible aggregation and decreased binding capacity.
参考图片
Immunoprecipitation of COX IV from HeLa cells using COX IV (3E11) Rabbit mAb #4850 and Protein A Magnetic Beads. Western blot analysis was performed on the 10% input control (lane 1), IP pellet (lane 2) and Rabbit (DAIE) mAb IgG XP® Isotype Control #3900 (at matched concentration) (lane 3) using COX IV (3E11) Rabbit mAb #4850.