The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.
商品描述
1A8
The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.
同种型
Rat LEW, also known as Lewis IgG2a, κ
克隆号
克隆 1A8 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen).
参考图片
Expression of Ly-6G on bone-marrow leukocytes. C57BL/6 bone-marrow cell suspensions were stained with either PE Rat Anti-Mouse Ly-6G (Cat. No. 551461, open histograms) or PE Rat IgG2a, κ Isotype Control (Cat. No. 553930, filled histograms), in the presence of Mouse Fc Block™ purified anti-mouse CD16/CD32 (Cat. No. 553141/553142). Non-viable leukocytes were excluded by staining with Propidium Iodide Staining Solution (Cat. No. 556463), and leukocyte subsets were distinguished by their light-scatter profiles. The left panel displays lymphoid and erythroid cells, and the right panel displays myeloid cells. Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).
Expression of Ly-6G on bone-marrow leukocytes. C57BL/6 bone-marrow cell suspensions were stained with either PE Rat Anti-Mouse Ly-6G (Cat. No. 551461, open histograms) or PE Rat IgG2a, κ Isotype Control (Cat. No. 553930, filled histograms), in the presence of Mouse Fc Block™ purified anti-mouse CD16/CD32 (Cat. No. 553141/553142). Non-viable leukocytes were excluded by staining with Propidium Iodide Staining Solution (Cat. No. 556463), and leukocyte subsets were distinguished by their light-scatter profiles. The left panel displays lymphoid and erythroid cells, and the right panel displays myeloid cells. Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).
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