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BD Pharmingen™ Human BD Fc Block™
品牌： BD Pharmingen
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对比
咨询反应种属：
Human (QC Testing), Rhesus (Tested in Development)
实验应用：
Flow cytometry (Routinely Tested)
产品介绍
产品信息
商品描述
Fc1
同种型
Human IgG1
克隆号
克隆 Fc1 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Human (QC Testing), Rhesus (Tested in Development)
目标/特异性
BD Fc Block™
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Nimmerjahn F, Ravetch JV. Fc-receptors as regulators of immunity. Adv Immunol. 2007; 96:179-204. (Biology).
2. Ravetch JV, Bolland S. IgG Fc receptors. Annu Rev Immunol. 2001; 19:275-290. (Biology).
参考图片
Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with BD Pharmingen™ Human BD Fc Block™. Human peripheral blood mononuclear cells were either not treated (dashed line histogram) or preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/ 564220; solid line histogram). The cells were then stained with an excess of irrelevant PE-conjugated Mouse IgG2a antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
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