BCA Protein Assay\\\\\\\\xa0Kit

BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution. How the assay works The BCA assay method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s). The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm. The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml). Support on how to determine protein concentration can be found here. Getting the best performance The BCA assay protocol is very flexible. Both the incubation time and temperature can be varied over a rather wide range. Lower protein samples can be more easily quantified using higher temperatures and longer incubation times. When assaying protein in solutions containing detergent, best results are obtained by adding the same amount of detergent to the wells containing the protein standard. BCA assay protocol summary - mix Copper Reagent and BCA Reagent to make BCA assay working solution - dispense samples and standards into 96-well plate - add working solution to each well - shake gently and incubate for 30-90 minutes - measure absorbance in a plate reader at 562 nm

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0.5 µg - 30 µg

Quantitative

Serum, Plasma, Cell culture extracts, Tissue Extracts

> 0.5 µg

BCA Bicinchoninic acid Bicinchoninic acid protein