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BMP-2 Quantikine PharmPak (50 Plates) (1 Pack)
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BMP-2 Quantikine ELISA Kit Summary
Sample Values
Cell Culture Supernates - Cell culture supernates from the following unstimulated cell lines were tested for natural BMP-2. No BMP-2 was detected.| Cell Lines | Type | Growth Conditions |
| ATDC5 | Mouse chondrogenic | :1 mixture of F-12 and DMEM + 5% FBS, L-glutamine, penicillin, and streptomycin |
| MCF-7 | Human breast cancer | 1:1 mixture of high glucose DMEM and F-12 + 10% FBS, L-glutamine, penicillin, and streptomycin |
| MDA-MB-453 | Human breast cancer | RPMI + 10% FBS, L-glutamine, penicillin, and streptomycin |
| ST-2 | Mouse-bone marrow stroma-cell derived | RPMI + 10% FBS, L-glutamine, penicillin, and streptomycin |
| U2OS | Human osteosarcoma | Grown to confluency in McCoy's 5a media + 15% FBS, L-glutamine, penicillin, and streptomycin |
Product Summary
Precision
Cell Culture Supernates, Bone Extracts
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 323 | 1010 | 2313 | 344 | 1043 | 2213 |
| Standard Deviation | 8.3 | 28.3 | 56 | 25.2 | 55.4 | 140.2 |
| CV% | 2.6 | 2.8 | 2.4 | 7.3 | 5.3 | 6.3 |
Recovery
The recovery of recombinant human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| 2 M Guanidine-HCI (n=1) | 98 | 94-101 |
| 2 M Urea (n=1) | 101 | 92-106 |
| Cell Culture Media (n=4) | 102 | 94-110 |
Linearity
Scientific Data
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Human/Mouse/Rat BMP-2 ELISA Standard Curve
Product Datasheets
Preparation and Storage
Background: BMP-2
BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.
Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.
Background: BMP-2
BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.
Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.