TMB Substrate Reagen (1 Pack)

INTENDED USE & DESCRIPTION

Substrate Reagent Pack contains 8 vials of Color Reagent A (stabilized hydrogen peroxide, Part # 895000) and 8 vials of Color Reagent B (stabilized tetramethylbenzidine, Part # 895001), both at 12 mL/vial, producing color development when contacting HRP-conjugated or biotinylated, streptavidin-HRP bound detection antibodies.   

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.

REAGENT PREPARATION

Warm to room temperature before use. Color Reagent A and Color Reagent B should be mixed together in equal volumes within 15 minutes of use. Protect from light. Add 100 µL of the mixture to each well and incubate at room temperature for 20 minutes while protecting from light. Add Stop Solution (Catalog # DY994) and read the microplate.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

• Caution: Color Reagent B may cause skin, eye, and respiratory irritation. Avoid breathing fumes. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use.