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Anti-mouse IgG (H+L), F(ab')2 Fragment was conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.
Product Usage Information
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500 - 1:2000 should yield acceptable results for immunofluorescent and flow cytometry assays.
Specificity/Sensitivity
Species Reactivity:
Mouse
Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
参考图片
Flow cytometric analysis of Jurkat cells using β-Actin (8H10D10) Mouse mAb #3700 (green solid line) compared to secondary antibody alone (green dashed line) or unstained cells (orange dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) was used as a secondary antibody.
Confocal immunofluorescent analysis of mouse olfactory bulb using Neurofilament-L (DA2) Mouse mAb #2835 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) #8890 (red) and Calbindin (D1I4Q) XP® Rabbit mAb #13176 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescent analysis of HeLa cells using β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).