ProLong®Gold Antifade Reagent with DAPI

ProLong Gold Antifade Reagent with DAPI offers enhanced resistance to photobleaching, is premixed and ready to use, and causes little or no quenching of the fluorescent signal. The reagent cures within 24 hours and samples can be saved for months after mounting. This reagent is compatible with most dyes and is appropriate for use in multi-color labeling assays.The inclusion of DAPI within this mounting reagent eliminates the need for a separate nuclear counterstaining step. 4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3). 1.Portugal, J. and Waring, M.J. (1988) Biochim Biophys Acta 949, 158-68. 2.Kapuscinski, J. (1995) Biotech Histochem 70, 220-33. 3.Manzini, G. et al. (1983) Nucleic Acids Res 11, 8861-76.