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Following immunostaining, cover samples with ProLong® Gold Antifade Reagent and coverslip.To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish.Proceed with imaging.
Store at -20°C. ProLong® Gold Antifade Reagent is stable for 12 months when stored, protected from light, at or below -20°C.
参考图片
Confocal immunofluorescent analysis of SNB19 cells using β-Actin (8H10D10) Mouse mAb #3700 (red), Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #9854 (green), and COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) #7561 (blue).
Confocal immunofluorescent analysis of HeLa cells using β-Actin (8H10D10) Mouse mAb #3700 (red) and COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853 (green). Blue pseudocolor = DRAQ5 #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of COS-7 cells, mounted in either glycerol (upper panels) or ProLong Gold Antifade Reagent (lower panels), using β-Actin (8H10D10) Mouse mAb #3700 and Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4409 (red) (0, 3.3, or 10 min of imaging).