ProLong ® Gold Antifade Reagent

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产品介绍

产品信息

简单描述

Buffer for studying Prolong Gold Anti-Fade Reagent in the research area.

商品描述

Product Usage Information

Following immunostaining, cover samples with ProLong® Gold Antifade Reagent and coverslip.To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish.Proceed with imaging.

背景

ProLong® Gold Antifade Reagent offers enhanced resistance to photobleaching, is premixed and ready to use, and causes little or no quenching of the fluorescent signal. The reagent cures within 24 hours and samples can be saved for months after mounting. This reagent is compatible with most dyes and is appropriate for use in multi-color labeling assays.

制备和贮存

保存方式

Store at -20°C. ProLong® Gold Antifade Reagent is stable for 12 months when stored, protected from light, at or below -20°C.

参考图片

Confocal immunofluorescent analysis of HeLa cells using β-Actin (8H10D10) Mouse mAb #3700 (red) and COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞，使用的抗体是：β-Actin (8H10D10) Mouse mAb #3700（红色）、COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853（绿色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

Confocal immunofluorescent analysis of SNB19 cells using β-Actin (8H10D10) Mouse mAb #3700 (red), Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #9854 (green), and COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) #7561 (blue).共聚焦免疫荧光分析SNB19细胞，使用的抗体是：β-Actin (8H10D10) Mouse mAb #3700（红色）、Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #9854（绿色）、COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) #7561（蓝色）。

Confocal immunofluorescent analysis of COS-7 cells, mounted in either glycerol (upper panels) or ProLong® Gold Antifade Reagent (lower panels), using β-Actin (8H10D10) Mouse mAb #3700 and Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4409 (red) (0, 3.3, or 10 min of imaging).共聚焦免疫荧光分析COS-7细胞，用甘油（上面）或ProLong® Gold Antifade Reagent（下面）封固，使用的抗体是：β-Actin (8H10D10) Mouse mAb #3700和Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4409（红色）(成像0, 3.3,或10分钟)。

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货号：

9071S

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10ml

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