PROTEOSTAT® Aggresome detection kit

Protect from light. Avoid freeze/thaw cycles.

The PROTEOSTAT® Aggresome Detection Kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. Featured In: High-throughput image-based aggresome quantification (SLAS Discov 2020; 25:783-791). Application: Optimized to monitor aggresome formation in a miniaturized, automated, and quantitative phenotypic assay. The approach is validated by screening a chemical library of 1280 compounds. Application Note: A Red-emitting Fluorescent Probe for Rapid Detection of Protein and Peptide Aggregates in Post-mortem Human Brain Tissue Sections from Patients Diagnosed with Alzheimer’s and Parkinson’s Disease Monitoring the Accumulation and Clearance of Exogenously Introduced Beta-amyloid in a Cell-Based Model of Alzheimer’s Disease by Fluorescence Microscopy and Fluorescence Microplate Assay Towards Understanding the Molecular Basis of Parkinson’s Disease: Cell-based Model of Mitophagy and Aggresome Accumulation Detection of bacterial aggregation by flow cytometry Cell-Based Screening of Focused Bioactive Compound Libraries: Assessing Small Molecule Modulators of the Canonical Wnt Signaling and Autophagy-Lysosome Pathways Monitoring Whole Brain Plaque Burden with PROTEOSTAT® Aggresome Detection Kit and Light Sheet Microscopy Cited samples: For an overview on cited samples please click here. Enzo and PROTEOSTAT® are trademarks of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.

Robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and  toxicology Provides a sensitive cell-based assay of drug responsiveness to identify inhibitors relevant to neurodegenerative disease in an authentic cellular context Reliable and simple assay doesn’t require non-physiological protein mutations or genetically engineered cell lines Validated under a wide range of conditions and with small molecule modulators demonstrating suitability for screening compounds of potential therapeutic value Fixed-cell assay is optimized for antibody co-localization studies Easily quantifies aggresome and related inclusion bodies by flow cytometry Useful for the study of neurodegenerative diseases, liver disease, toxicology studies and much more Aggresomes are inclusion bodies of aggregated, misfolded proteins that form when the ubiquitin-proteasome protein-degradation machinery is overwhelmed. Typically, aggresomes form in response to cellular stress and provide a cytoprotective mechanism by isolating misfolded proteins, leading ultimately to their clearance by autophagy. Protein aggregate formation is furthermore a hallmark of a variety of human diseases, such as Alzheimer’s, Parkinson’s, Amyotrophic lateral sclerosis or alcoholic liver disease. The PROTEOSTAT® Aggresome Detection Reagent contained in this kit is a molecular rotor dye. While in solution, free intramolecular rotation along a single central bond prevents fluorescence. The PROTEOSTAT® dye specifically intercalates into the cross-beta spine of quaternary protein structures typically found in misfolded and aggregated proteins, which will inhibit the dye’s rotation and lead to a strong fluorescence. PROTEOSTAT® Aggresome Detection kit provides a rapid, specific and quantitative approach to label aggresomes in cells, eliminating the need of artificial protein mutations. PROTEOSTAT® dye has been validated under a wide range of conditions and with small molecule modulators, demonstrating its suitability for compound screening. Furthermore, it is suitable for multiplex co-immunofluorescence to study your target of interest in the context of autophagy and aggresome formation.

Flow Cytometry, Fluorescence microscopy, Fluorescent detection;The PROTEOSTAT® Aggresome detection kit assay is potentially applicable to the identification of small molecules that inhibit aggresome formation as well as immuno-localization studies between aggregated protein cargo and the various proteins implicated in aggresome

With proper storage, the kit components are stable for one year from date of receipt.