ROS-ID® Total ROS/Superoxide detection kit

Protect from light. Avoid freeze/thaw cycles.

The ROS-ID® Total ROS/Superoxide detection kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLELSTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. Toxicology Application Note:Use of 3D Cultured Human iPSC-Derived Hepatocytes for Long-Term Hepatotoxicity StudiesScreening Reactive Oxygen Species (ROS) on IQUE® Screener Cited samples:For an overview on cited samples please click here.

Widely cited kit to detect total ROS and Superoxide in live cells by microscopy and flow cytometry applications Specifically designed to measure Superoxide production in live cells Compatible with major components of tissue culture media (phenol red, FBS and BSA) Validated on microscopy, flow cytometry, and microplates Enzo Life Sciences ROS-ID® Total ROS/Superoxide detection kit includes dyes to measure Reactive Oxygen Species (ROS) and Superoxide production in live cells. The non-fluorescent, cell-permeable Oxidative Stress Detection Reagent (Green, Ex/Em 490/525 nm) reacts directly with a wide range of reactive species (hydrogen peroxide, peroxynitrite and hydroxyl radicals) yielding a green fluorescent product. The Superoxide Detection Reagent (Orange, Ex/Em 550/620 nm) is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. Pyocyanin (ROS inducer) and NAC (ROS inhibitor) are also provided with the kit.

Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS;This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.

Reactive oxygen species / Superoxide

With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.