UBIQAPTURE-Q® kit

Market-leading tool for isolation and detection of ubiquitinylated proteins. Efficient tool for the selective isolation of ubiquitinylated proteins High binding affinity matrix, able to bind both monoubiquitinated and polyubiquitinated proteins Highly compatible with a wide range of lysate buffer, different sample types (cells, tissues) and a broad range of species For isolation and enrichment of ubiquitinylated proteins.The UBI-QAPTURE-Q® kit is an efficient tool for the selective isolation of ubiquitinylated proteins. The Kit facilitates the isolation of both mono- and poly-ubiquitinylated proteins (independent of lysine residue chain linkage) from cell extracts, tissue lysates and in vitro assay solutions through use of a high-binding affinity matrix. Captured proteins can be analyzed by Western blotting using the highly sensitive ubiquitin-conjugate specific antibody provided, using antibodies to specific proteins of interest, or eluted from the matrix for subsequent biochemical characterization. The UBI-QAPTURE-Q® matrix supplied with the kit has superior binding characteristics compared to other commercially available matrices and is compatible with a wide range of lysate buffers and cell/tissue samples from a variety of species.

Uses: Isolation and detection of the full range of ubiquitinylated protein conjugates (mono-/multi-/poly-ubiquitin modified, lysine linkage independent) from specific cell/tissue lysates of interest, especially low abundance (regulatory) proteins involved in the ubiquitin-proteasome pathway. Capture and analysis of specific

Upon receipt, the control lysate (Prod. No. BML-UW0130) should be stored at -80°C. All other components should be stored at 4°C.