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PGE2 Assay Kit (1 Kit)
品牌： R&D Systems
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Product Summary
The Parameter PGE2 Immunoassay is a 3.5 hour forward sequential competitive enzyme immunoassay designed to measure PGE2 in cell culture supernates, serum, plasma, and urine.
Recovery
The recovery of PGE2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 84-106 |
| EDTA Plasma (n=4) | 93 | 82-108 |
| Heparin Plasma (n=4) | 94 | 81-111 |
| Serum (n=4) | 97 | 86-114 |
| Urine (n=4) | 99 | 81-111 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of PGE2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Prostaglandin E2 Parameter Assay Kit Summary
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (51 uL), Serum (51 uL), EDTA Plasma (51 uL), Heparin Plasma (51 uL), Urine (51 uL)
Sensitivity
41.4 pg/mL
Assay Range
39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Measures PGE2 levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
背景
别名
Dinoprostone,PGE2
背景
Background: Prostaglandin E2/PGE2
Prostaglandin E2 (PGE2) is formed in a variety of cells from prostaglandin H2, which is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of human immunodeficiency virus replication. It elevates cAMP levels, stimulates bone resorption and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.
Alternate Names:
Dinoprostone; PGE2
研究领域
制备和贮存
保存方式
Preparation and Storage
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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