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简单描述
When diluted in lysis buffer to a final concentration of 1X the Protease Inhibitor Cocktail prevents protein degradation by endogenous proteases present in whole cell extract. The 100X Protease Inhibitor Cocktail is a clear, colorless liquid.
1X Protease Inhibitor Cocktail: 1mM AEBSF800nM Aprotinin50μM Bestatin15μM E-6420μM Leupeptin10μM Pepstatin A
商品描述
Product Usage Information
1. Briefly vortex the Protease Inhibitor Cocktail (100X) before use.2. Just prior to lysing cells, dilute the cocktail 1:100 in desired lysis buffer to obtain a 1X working concentration.
应用
实验应用
IP
背景
背景
In order to study specific target proteins of interest protease-mediated degradation during the generation of protein lysates is to be avoided. A loss of normal cellular control occurs during cell lysis, and endogenous proteases within the cell extract are free to degrade proteins in an uncontrolled manner. The addition of protease inhibitors to the cell lysis buffer aids in the preservation of target proteins in the cell extract.
翻译后修饰
unmodified
制备和贮存
保存方式
Store the undiluted 100X cocktail at 4ºC. Do not freeze.
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