Protein G Agarose Beads

Protein G Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein G is covalently coupled to agarose beads. Protein G exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies.

Product Specifications:

Bead Diameter: ~50-150 micron per bead

Binding Capacity: ~20 mg human IgG/ml

Product Usage Information

Vortex tube briefly to resuspend the beads. Add 20-40 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST's recommended IP protocol to perform IP followed by western blot.

Specificity/Sensitivity

Species Reactivity:

All Species Expected