




Product Usage Information
Vortex tube briefly to resuspend the beads. Add 20-40 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST's recommended IP protocol to perform IP followed by western blot.




参考图片
Immunoprecipitation (IP) of COX IV from HeLa cells using COX IV (4D11-B3-E8) Mouse mAb #11967 and Protein G Agarose Beads. Western blot analysis was performed on the IP pellet (lane 1) and supernatant (lane 2) using COX IV (3E11) Rabbit mAb #4850.
Immunoprecipitation (IP) of Akt from 3T3 cells using Akt (pan) (40D4) Mouse mAb #2920 and Protein G Agarose Beads. Western blot analysis was performed on the IP pellet (lane 1) and supernatant (lane 2) using Akt (pan) (C67E7) Rabbit mAb #4691.