老带新
hAngiogenesis Array (1 Kit)
1/3

hAngiogenesis Array (1 Kit)

分享
品牌: R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序,查商品更便捷
收藏
对比对比
咨询咨询
产品介绍
产品介绍
产品信息
试剂盒组分
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Antibody Detection Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

Scientific Data

Profiling Angiogenesis-Related Proteins in Human Cancer Tissues. A) The Proteome Profiler Human Angiogenesis Antibody Array (Catalog # ARY007) was used to profile angiogenesis-related proteins in tissues from human prostate, ovarian, and breast cancers. B) Histogram profiles for select analytes were generated by quantifying the mean spot pixel densities from the array membrane using image software.

The Human Angiogenesis Array detects multiple analytes in cell culture supernates. A. THP-1 human acute monocytic leukemia cell line treated with 1 µg/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 8 hours and 1 µg/mL lipopolysaccharide (LPS) for 16 hours. B. HUVEC human umbilical vein endothelial cells treated with 10 mg/ml of LPS for 24 hours. 500 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

The Human Angiogenesis Array detects multiple analytes in tissue lysate, serum, and plasma. Representative membranes and selective quantification are shown for human ovarian cancer tissue lysate (A., 200 µg), serum (B., 100 µL), and EDTA plasma (C., 100 µL). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Proteome Profiler Human Angiogenesis Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected angiogenesis-related proteins. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma and saliva.

Troubleshooting Guide

Key Benefits

  • Detects 55 human angiogenesis-related proteins simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are rquired.  Refer to protocol.

Principle of the Assay

The Proteome Profiler Human Angiogenesis Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of bound analyte. Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules.

Why Use an Antibody Array to Detect Angiogenesis-Related Proteins?

Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Antibody Detection Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 
Simultaneously detect the relative levels of these angiogenesis-related proteins in a single sample.
Activin AFGF-7/KGFPD-ECGF
ADAMTS-1GDNFPDGF-AA
AngiogeninGM-CSFPDGF-AB/PDGF-BB
Angiopoietin-1HB-EGFPersephin
Angiopoietin-2HGFCXCL4/PF4
Angiostatin/PlasminogenIGFBP-1PlGF
AmphiregulinIGFBP-2Prolactin
ArteminIGFBP-3Serpin B5/Maspin
Tissue Factor/Factor IIIIL-1 betaSerpin E1/PAI-1
CXCL16CXCL8/IL-8Serpin F1/PEDF
DPPIV/CD26LAP (TGF-beta 1)TIMP-1
EGFLeptinTIMP-4
EG-VEGFCCL2/MCP-1Thrombospondin-1
Endoglin/CD105CCL3/MIP-1 alphaThrombospondin-2
Endostatin/Collagen XVIIIMMP-8uPA
Endothelin-1MMP-9Vasohibin
FGF acidicNRG1-beta 1VEGF
FGF basicPentraxin 3VEGF-C
FGF-4  

 

Assays for analytes represented in the Human Angiogenesis Antibody Array Kit

 Quantikine® ELISA KitsDuoSet® ELISA Development SystemsQuantikine® HS (High Sensitivity) ELISA KitsQuantiGlo® Chemiluminescent ELISA Kits
Activin ADAC00BDY338  
ADAMTS-1 DY2197  
AngiogeninDAN00DY265  
Angiopoietin-1DANG10DY923  
Angiopoietin-2DANG20DY623  
Angiostatin/Plasminogen    
Amphiregulin DY262  
Artemin DY2589  
Tissue Factor/Factor IIIDCF300DY2339  
CXCL16DCX160DY1164  
DPPIV/CD26 DY1180  
EGFDEG00DY236  
EG-VEGF DY1209  
EndoglinDNDG00DY1097  
Endostatin/Collagen XVIIDNST0DY1098  
Endothelin-1DET100  QET00B
FGF acidicDFA00BDY232  
FGF basicDFB50DY233HSFB00D 
FGF-4 DY235  
FGF-7/KGFDKG00DY251  
GDNF DY212  
GM-CSFDGM00DY215HSGM0 
HB-EGF DY259  
HGFDHG00DY294  
IGFBP-1 DY871  
IGFBP-2DGB200DY674  
IGFBP-3DGB300DY675  
IL-1 betaDLB50DY201HSLB00DQLB00B
CXCL8/IL-8D8000CDY208HS800Q8000B
TGF-beta1 (LAP)DB100BDY240  
LeptinDLP00DY398  
CCL2/MCP-1DCP00DY279  
CCL3/MIP-1 alphaDMA00DY270  
MMP-8DMP800DY908  
MMP-9DMP900DY911  
NRG1-beta1 DY377  
Pentraxin 3DPTX30DY1826  
PD-ECGF    
PDGF-AADAA00BDY221  
PDGF-AB/PDGF-BBDHD00C, DBB00DY222, DY220  
Persephin    
CXCL4/PF4 DY795  
PlGFDPG00DY264  
ProlactinDPRL00DY682  
Serpin B5/Maspin    
Serpin E1/PAI-1DSE100DY1786  
Serpin F1/PEDF    
TIMP-1DTM100DY970  
TIMP-4DTM400DY974  
Thrombospondin-1DTSP10DY3074  
Thrombospondin-2DTSP20DY1635  
uPADUPA00DY1310  
Vasohibin    
VEGFDVE00DY293B QVE00B
VEGF-CDVEC00DY752B  

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human
声明 :本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。
货号:
ARY007
一键复制
询价
1Kit
选择数量
当前规格1件起购 
配送至
预计2-3周送达,快递: 免运费,若需干冰额外收费