PROTEOSTAT® Protein aggregation assay

Application Notes:Particle analysis of therapeutic protein formulations with ImageStreamX® Imaging Flow Cytometry and the PROTEOSTAT® Protein Aggregation Assay Prediction of Aggregation Propensity and Monitoring of Aggregation of Antibody-Drug Conjugates (ADC) using ProteoStat® Reagents Enzo and PROTEOSTAT are trademarks of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. Cited samples:PROTEOSTAT® Cited Samples

Quantitative Detection of  Protein Aggregates from Visible to Subvisible Particles A simple, sensitive, homogenous fluorescent assay Validated for use with microplate or flow cytometry platform Extensively benchmarked with IgG Optimize buffers and excipients for protein formulation Performs with a wide pH and ionic strength range Use with PROTEOSTAT® Protein Aggregation Standards for accurate quantification of aggregated protein in solution. PROTEOSTAT® Protein aggregation assay provides a simple,  homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures. Relative to conventional protein aggregation detection dyes, such as Thioflavin T, the Enzo PROTEOSTAT® detection reagent can detect aggregates from a broader range of proteins, yields a much brighter signal, provides at least 2 orders of magnitude linear dynamic range and offers superior performance across a broad range of pH values (4~10) and buffer compositions. Sensitivity for this assay is in the sub-micromolar range and as little as 1-5% protein aggregate is detectable in a concentrated protein solution. The assay is capable of providing quantitative analysis of protein aggregation in a robust and high-throughput fashion (Z’ factor score >0.5). Lyophilized native and aggregated protein are provided as negative and positive controls for monitoring changes in protein aggregation status. PROTEOSTAT® Protein Aggregation Standards kit (Prod. No. ENZ-51039) is the only commercially available protein aggregation standards assay with stabilized, high-quality reference samples for generating trace protein aggregate levels in concentrated monomeric IgG.Easy to use – simply add water!

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Flow Cytometry, Fluorescence microscopy, Fluorescent detection, Microplate;This kit has been specifically designed for monitoring of protein aggregate formation in solution.

With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer or -80°C for longer term storage.