PTEN and PDK1 Antibody Sampler Kit II

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PTEN and PDK1 Antibody Sampler Kit II

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

PTEN and PDK1

简单描述

The PTEN and PDK1 Antibody Sampler Kit II provides an economical means to evaluate two key enzymes that regulate multiple signaling pathways. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

背景

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (11,12), including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (13). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation, and apoptosis. 1.Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. 2.Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7. 3.Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8. 4.Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11. 5.Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91. 6.Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8. 7.Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8. 8.Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30. 9.Funamoto, S. et al. (2002) Cell 109, 611-23. 10.Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610. 11.Belham, C. et al. (1999) Curr Biol 9, R93-6. 12.Toker, A. and Newton, A.C. (2000) Cell 103, 185-8. 13.Williams, M.R. et al. (2000) Curr Biol 10, 439-48.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

翻译后修饰

phosphate

数据库链接

Entrez-Gene ID

5170,5728

UniProt ID

O15530,P60484

参考图片

Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using PTEN (D4.3) XP® Rabbit mAb performed on the Leica BOND RX.

Western blot analysis of extracts from PC3 cells, HCT116 wild-type and HCT116 PDK1 -/- cells using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT116 wild-type and HCT116 PDK1 -/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

Western blot analysis of extracts from various cell lines using PDK1 (D37A7) Rabbit mAb.

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western blot analysis of extracts from various cell lines using PTEN (D4.3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Immunohistochemical analysis of paraffin-embedded human colon using PTEN (D4.3) XP® Rabbit mAb.

Immunohistochemical analysis using PTEN (D4.3) XP® Rabbit mAb on SignalSlide(TM) PTEN IHC Controls #8106 (paraffin-embedded LNCaP (left) and NIH/3T3 (right) cells).

Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using PTEN (D4.3) XP® Rabbit mAb #9188. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using PTEN (D4.3) XP® Rabbit mAb performed on the Leica BOND RX.

Western blot analysis of extracts from HeLa and NIH/3T3 cells, using Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb. Membranes were either left untreated (-) or treated with (+) calf intestinal phosphatase (CIP) post Western transfer to verify phospho-specificity of the antibody.

Western blot analysis of extracts from PC3 cells, untreated or λ phosphatase-treated, using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper), PDK1 Antibody #3062 (middle) or Akt Antibody #9272 (lower).

Western blot analysis of extracts from HCT 116 wild-type and HCT 116 PDK1-/- cells using PDK1 (D37A7) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT 116 wild-type and HCT 116 PDK1-/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using PTEN (D4.3) XP® Rabbit mAb performed on the Leica BOND RX.

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货号：

30590T

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