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PTMScan ® HS Phospho-Tyrosine (P-Tyr-1000) Kit
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咨询PTMScan® HS is an enhanced PTMScan® methodology with improved identification of post-translationally modified peptides. PTMScan® technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography tandem mass spectrometry (LC-MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. PTMs that can be analyzed by PTMScan® technology include phosphorylation, ubiquitination, acetylation, and methylation, among others. The technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity (HS), providing a global overview of PTMs in cell and tissue samples without bias about where the modified sites occur. For more information on PTMScan® products and services, please visit www.cellsignal.com/applications/proteomics.
All components in this kit are stable for at least 12 months when stored at the recommended temperature. Do not aliquot the antibody.
参考图片
Motif analysis using all phospho-tyrosine peptides enriched and identified by PTMScan® HS Phospho-Tyrosine (P-Tyr-1000) Kit from two different samples. Peptides from mouse embryo and MKN-45 human gastric cancer cells were immunoprecipitated with PTMScan® HS pY Magnetic Immunoaffinity Beads in seven independent experiments. Orbitrap Fusion™ Lumos™ mass spectrometer analysis identified a total of 3,620 unique sites. The motif logo shows that the pY antibody is a general motif antibody that recognizes the pY motif independent of protein context, without other amino acid preferences.