QIAprep 96 Plus Miniprep Kit (4)

• Consistently high yields of up to 50 µg
• Pure preps suitable for a wide range of applications
• Vacuum, centrifugation, and automated processing options

QIAprep 96 Plus Kits enable plasmid preparation in 96-well format. Samples can be processed using QIAvac 96 and/or a centrifuge or automated on the BioRobot Universal System. Up to 50 µg of high-quality plasmid DNA (endotoxin levels <10 EU/µg DNA) can be obtained from up to 5 ml of culture.
For optimal results it is recommended to use this product together with QIAvac 96.

High-purity preps and outstanding yields

QIAprep 96 Plus Kits provide a unique method for preparation of highly pure DNA (see figure " Superior plasmid DNA quality"). Yields of up to 50 µg can be consistently achieved using an optimized procedure. The unique binding buffer provided with the kits ensures very specific binding conditions (see figures " High yields of plasmid DNA" and " Superior performance compared to other suppliers").

The unique kit chemistry and design of the Plasmid Plus 96 plates allow high binding capacities. After neutralization, a TurboFilter 96 plate is used for lysate clearing. To optimize plasmid DNA binding to the membrane of the Plasmid Plus 96 plate, a nonchaotropic binding buffer is added to the cleared lysate. The unique binding buffer ensures very specific binding conditions, resulting in DNA quality that is comparable to anion-exchange preps (see figure " Superior plasmid DNA quality"). Due to unique kit chemistry, up to 50 µg plasmid DNA per well can be obtained from a 5 ml E. coli culture (see figures " High yields of plasmid DNA" and " Superior performance compared to other suppliers").

QIAprep 96 Plus Kits allow plasmid preparation in 96-well format. The QIAprep 96 Plus Miniprep protocol is based on a modified alkaline lysis procedure. Following lysate clearing, a simple bind-wash-elute procedure results in highly concentrated plasmid DNA, ready for direct use in subsequent applications (see flowchart " QIAprep 96 Plus Miniprep procedure").

Convenient and flexible processing

The QIAprep 96 Plus Miniprep Kit can be conveniently processed using the QIAvac 96 and/or a centrifuge, while preps can be automated on the BioRobot Universal System using the QIAprep 96 Plus BioRobot Kit. Optimized protocols ensure easy and straightforward plasmid DNA preparation in 96-well format.

Plasmid DNA purified using QIAprep 96 Plus Kits is highly suitable for a wide range of applications, including:

• Preparation of short hairpin vectors (sh-vectors)
• Enzymatic modifications
• Cloning
• Restriction digestion
• Sequencing