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Rabbit anti-ADRP/Perilipin-2 Recombinant Monoclonal Antibody(S-2210-3)
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PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
参考图片
WB resuLt of ADRP/Perilipin-2 Recombinant Rabbit mAb
Primary antibody: ADRP/Perilipin-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Low expression control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa
WB resuLt of ADRP/Perilipin-2 Recombinant Rabbit mAb
Primary antibody: ADRP/Perilipin-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa
ICC shows positive staining in HepG2 cells. Anti-ADRP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubuLin (Red).