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Rabbit anti-Cleaved-caspase3 Polyclonal Antibody
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品牌： Absin
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对比
咨询分子量：
Predicted molecular weight: 32kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
反应种属：
Human;Mouse;Rat
产品介绍
产品信息
荧光素标记
宿主
Rabbit
免疫原
The antiserum was produced against synthesized peptide derived from human Caspase 3.
分子量
Predicted molecular weight: 32kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
克隆类别
Polyclonal Antibody
浓度
1mg/ml
应用
实验应用
WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:100, ICC: 1:100-1:500
反应种属
Human;Mouse;Rat
背景
翻译后修饰
Unmodified
细胞定位
Cytosol;Nucleus(cleaved);Plasma Membrane
制备和贮存
存储溶液
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
保存方式
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
参考图片
Western blot analysis of extracts from various samples, using Cleaved-Caspase 3 (Asp175), p17 Ab. Lane 1: Rat liver, blocked with antigen-specific peptides. Lane 2: Rat liver. Lane 3: Rat spleen.
abs132005 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).
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