Rabbit anti-FAP Recombinant Monoclonal Antibody(R132)

WB result of FAP Rabbit mAb Primary antibody: FAP Rabbit mAb at 1/1000 dilution Lane 1: SK-MEL-28 whole cell lysate 20ug Lane 2: HFF whole cell lysate 20ug Low expression control: SK-MEL-28 whole cell lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 87 kDa Observed MW: 95 kDa Exposure time: 30s

IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-FAP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-FAP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FAP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-FAP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.