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Rabbit anti-GAPDH Polyclonal Antibody
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咨询Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
参考图片
Sample: Lane 1,4,7: Mouse Cerebellum tissue lysates Lane 2,5,8: Rat Cerebellum tissue lysates Lane 3,6,9: Human SH-SY5Y cell lysates Primary: Anti-GAPDH at 1/10000~1/100000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 36 kDa
Sample: Lane 1: Cerebellum (Mouse) Lysate Lane 2: Raw264.7 (Mouse) Cell Lysate Lane 3: NIH/3T3 (Mouse) Cell Lysate Lane 4: Cerebrum (Rat) Lysate Lane 5: SH-SY5Y (Human) Cell Lysate Lane 6: MCF-7 (Human) Cell Lysate Primary: Anti-GAPDH at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit,) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.