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WB resuLt of Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb
Primary antibody: Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 starve 18 hours, then treated with 50 ng/mL PDGF for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 68 kDa
WB resuLt of Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb
Primary antibody: Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 68 kDa