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Rabbit anti-α-Synuclein Recombinant Monoclonal Antibody(S-441-86)
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Rabbit anti-α-Synuclein Recombinant Monoclonal Antibody(S-441-86)

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品牌: Absin
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来源宿主:
Rabbit
实验应用:
WB: 1:1000, IP: 1:50, IF: 1:1000
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Protein A
宿主
Rabbit
免疫原
Recombinant Protein
克隆号
S-441-86
克隆类别
Monoclonal Antibody
浓度
0.5 mg/mL
应用
实验应用
WB: 1:1000, IP: 1:50, IF: 1:1000
背景
翻译后修饰
Unmodified
细胞定位
Cytoplasm,Nucleus,Membrane
制备和贮存
存储溶液
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
保存方式
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

参考图片

WB result of α-Synuclein Rabbit mAb Primary antibody: α-Synuclein Rabbit mAb at 1/1000 dilution Lane 1: mouse brain lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 14 kDa Observed MW: 18 kDa Exposure time: 120 s

WB result of α-Synuclein Rabbit mAb Primary antibody: α-Synuclein Rabbit mAb at 1/1000 dilution Lane 1: rat brain lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 14 kDa Observed MW: 18 kDa Exposure time: 180 s

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse testis. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-α-Synuclein antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

α-Synuclein Rabbit mAb at 1/50 dilution (1 ug) immunoprecipitating α-Synuclein in 0.4 mg mouse brain lysate. Western blot was performed on the immunoprecipitate using α-Synuclein Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: mouse brain lysate 20 ug (Input) Lane 2: α-Synuclein Rabbit mAb IP in mouse brain lysate Lane 3: Rabbit monoclonal IgG IP in mouse brain lysate Predicted MW: 14 kDa Observed MW: 18 kDa Exposure time: 90 s

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货号:
abs173485-25uL
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