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Rabbit anti-Cleaved LC3A Polyclonal Antibody
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咨询Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
参考图片
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized NIH/3T3 ( Mouse mouse embryonic fibroblasts cell line) cells labeling Pdx1 with abs103336 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). The nuclear counter stain is DAPI (blue). Immunofluorescence image showing cytoplasm on NIH/3T3 cell line.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with abs103336 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Immunofluorescence image showing vesicles staining on Hela cell line. The nuclear counter stain is DAPI (blue). The right image is Hela cells treated with Chloroquine 50μM for 16h.
abs103336 staining Cleaved-APG8a in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) Antibody (Cat. #abs103336)(upper) or Beta-actin (lower).
Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A)(RB52604)(upper) or Beta-actin (lower).
Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A)(Cat. #abs103336)(upper) or Beta-actin (lower).
Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A)(Cat. #abs103336)(upper) or Beta-actin (lower).