Rabbit Polyclonal Rabbit anti-Sheep IgG Fc Secondary Antibody [HRP] (Azide Free)

Ion exchange chromatography

Rabbit

Purified normal IgG isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

LYOPH

No Preservative

Polyclonal

Dot Blot 1:100-1:2000ELISA 1:100-1:2000Immunoblotting 1:100 - 1:2000Immunocytochemistry/ Immunofluorescence 1:10 - 1:500Immunohistochemistry-Paraffin Western Blot

The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in sheep serum, as tested by immunoelectrophoresis and double radial immunodiffusion.