Rag and LAMTOR Antibody Sampler Kit

The mTORC kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). According to the research literature, RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered to be a viable target for further diagnostic and therapeutic study (5). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit four Rag GTPases to the surface of lysosomes (6). 1.Sancak, Y. et al. (2008) Science 320, 1496-501. 2.Kim, E. et al. (2008) Nat Cell Biol 10, 935-45. 3.Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. 4.Wullschleger, S. et al. (2006) Cell 124, 471-84. 5.de Wit, N.J. et al. (2005) Br J Cancer 92, 2249-61. 6.Sancak, Y. et al. (2010) Cell 141, 290-303.