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咨询The Rapid-Act T Cell Activation Kit (Mouse, Anti-CD3/CD28) provides the necessary reagents for easy, single-step induction of mouse T cell activation and proliferation. The included Synthetic Immune Synapse particles pre-cluster the CD3 and CD28 antibodies for thorough T cell activation, and are small enough to minimize cell clumping. Because the reagents are added to the cells in a single step, the kit enables accurate time course studies for short-term T cell stimulation. The kit can also be used for longer-term T cell proliferation and exhaustion assays. No modification or removal is necessary prior to subsequent analysis by flow cytometry.
Specificity/Sensitivity
Species Reactivity:
Mouse
Store at 4ºC. All components in this kit are stable for 12 months when kept in the original format and stored at 4ºC. Once reconstituted, store the Anti-mouse CD3 and Anti-mouse CD28 at -80ºC. Aliquot to avoid multiple freeze/thaw cycles.
参考图片
Flow cytometric analysis of mouse splenocytes, untreated (left column) or treated with Rapid-Act T Cell Activation Kit (Mouse, Anti-CD3/CD28) (15 min; right column), using Phospho-SLP-76 (Ser376) (E3G9U) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #47876 (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (bottom row), and co-stained with CD3 (17A2) Rat mAb (APC Conjugate) #24265.