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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu37 of human K-Ras protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #82819.
参考图片
Immunohistochemical analysis of paraffin-embedded BEN cell pellet (left, higher-expressing) or MDA-MB-468 cell pellet (right, lower-expressing) using Ras (E8N8L) XP® Rabbit mAb.
Western blot analysis of extracts from COS-7 cells, untransfected or transfected with a construct expressing MycDDK tagged K-Ras, H-Ras, or N-Ras, and extracts from mouse brain or ovary tissues using Ras (E8N8L) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using Ras (E8N8L) XP® Rabbit mAb.
Simple Western™ analysis of lysates (0.1 mg/mL) from COS-7 cells using Ras (E8N8L) XP® Rabbit mAb #67648. The virtual lane view (left) shows a single target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Immunoprecipitation of Ras protein from SW620 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Ras (E8N8L) XP® Rabbit mAb. Western blot analysis was performed using Ras (E8N8L) XP® Rabbit mAb. Anti-rabbit IgG HRP-linked Antibody #7074 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded normal human colon using Ras (E8N8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using Ras (E8N8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Ras (E8N8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human kidney using Ras (E8N8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Ras (E8N8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse stomach using Ras (E8N8L) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse brain using Ras (E8N8L) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).