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Rat Gastrin (GT) ELISA Kit

产品介绍
产品介绍
产品信息
抗原名称
gastrin

商品描述
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GT in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GT has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled GT and unlabeled GT (Standards or samples) with the pre-coated antibody specific to GT. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GT in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GT in the sample.

应用
反应种属
Rat

检测范围
15.625-1000pg/mL

检测类型
Competitive inhibition

样本类型以及上样量
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

敏感性
4.63pg/mL

背景
别名
GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III

制备和贮存
保存方式
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
数据库链接
NCBI
25320

UniProt ID
P04563
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